Abstract:In this study, nine kinds of Chinese herbal medicines, such as Epimedium brevicornu Maxim, Cistanche deserticola Ma and actinolite, were fermented by Saccharomyces cerevisiae and Bacillus subtilis to prepare Chinese herbal compound. 24 healthy young China Holstein were randomly divided into three groups(8 cows each group): control group(fed with basic diet), 1% experimental group(fed with basic diet+1% Chinese herbal compound) and 2% experimental group(fed with basic diet+2% Chinese herbal compound) and then were treated with the Chinese herbal compound. The feed intake and serum related indexes of each group were measured, the reproductive function indexes of each group were recorded, and the effects of Chinese herbal compound on feed intake, antioxidant capacity and reproductive performance were analyzed. The results showed that the addition of Chinese herbal compound in the basal diet could significantly increase the dry matter intake of China Holstein(P<0.01), in addition to the 2% experimental group on the 14th to 7th day before delivery. In the aspect of antioxidant capacity, compared with the control group, the catalase activity in the serum of 1% treatment group decreased obviously at 14 days before delivery and 21 days after delivery(P<0.05); the malondialdehyde(MDA) content in the serum of 1% treatment group decreased significantly at 7 days before delivery and on the day of calving(P<0.01) and it decreased obviously on the 14th day after delivery(P<0.05); the MDA content in the serum of 2% treatment group decreased obviously at 7 days before delivery(P<0.05) and it decreased significantly at the day of calving and the 14th day after calving(P<0.01). Regarding to the reproductive hormone, compared with the control group, the estradiol(E2) content in the serum of the 2% experimental group decreased obviously on the 21th day before delivery(P<0.05), decreased significantly on the 14th day before delivery(P<0.01), and increased significantly on the 7th day before delivery(P<0.01). For the cow reproduction, compared with the control group, the incidence of endometritis and retained placenta in the 2% experimental group was lower, and there was no mastitis, and the first mating time were advanced 15 days. It can be seen that adding probiotics fermented Chinese herbal compound in the diet could significantly improve the intake of China Holstein, reduce the incidence of postpartum diseases, promote postpartum estrus of China Holstein, and advance the first breeding time. Adding 2% Chinese herbal compound to basal diet showed optimal effect.