发酵中草药复合物对奶牛抗氧化和繁殖性能的影响
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国家重点研发计划项目(2017YFD0502005)、湖南省重点研发计划项目(2017NK2351)、创新平台与人才计划–湖南省科技特派员创新创业项目(2019NK4202)


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    摘要:

    利用酿酒酵母菌和枯草芽孢杆菌发酵淫羊藿、肉苁蓉、阳起石等9种中草药,制备中草药复合物,选用24头健康的荷斯坦青年奶牛,随机分为3组(8头/组),分别为对照组(饲喂基础日粮)、1%试验组(饲喂基础日粮+1%中草药复合物)和2%试验组(饲喂基础日粮+2%中草药复合物),测定和记录各组奶牛的采食量、血清相关指标及繁殖功能指标,分析中草药复合物对奶牛采食量、抗氧化能力和繁殖性能的影响。结果表明:除产前第14~7天的2%试验组外,基础日粮中添加中草药复合物可极显著提高奶牛干物质采食量(P<0.01);抗氧化能力方面,与对照组相比,1%试验组奶牛血清过氧化氢酶活力在产前第14天、产后第21天显著下降(P<0.05),1%试验组奶牛血清丙二醛含量在产前第7天、产犊当天极显著下降(P<0.01),产后第14天显著下降(P<0.05),2%试验组奶牛血清丙二醛含量在产前第7天显著下降(P<0.05),在产犊当天、产后第14天极显著下降(P<0.01);生殖激素方面,与对照组相比,2%试验组奶牛血清雌二醇含量在产前第21天显著下降(P<0.05)、产前第14天极显著下降(P<0.01)、产前第7天极显著上升(P<0.01);奶牛繁殖方面,与对照组相比,2%试验组奶牛的子宫内膜炎、胎衣不下发生率低且无乳房炎发生,产后首次配种时间提前15 d。可见,在基础日粮中添加益生菌发酵中草药复合物能明显提高奶牛采食量,降低产后疾病发生率,促进母牛产后发情,从而使首次配种时间提前。以基础日粮中添加2%中草药复合物的效果较好。

    Abstract:

    In this study, nine kinds of Chinese herbal medicines, such as Epimedium brevicornu Maxim, Cistanche deserticola Ma and actinolite, were fermented by Saccharomyces cerevisiae and Bacillus subtilis to prepare Chinese herbal compound. 24 healthy young China Holstein were randomly divided into three groups(8 cows each group): control group(fed with basic diet), 1% experimental group(fed with basic diet+1% Chinese herbal compound) and 2% experimental group(fed with basic diet+2% Chinese herbal compound) and then were treated with the Chinese herbal compound. The feed intake and serum related indexes of each group were measured, the reproductive function indexes of each group were recorded, and the effects of Chinese herbal compound on feed intake, antioxidant capacity and reproductive performance were analyzed. The results showed that the addition of Chinese herbal compound in the basal diet could significantly increase the dry matter intake of China Holstein(P<0.01), in addition to the 2% experimental group on the 14th to 7th day before delivery. In the aspect of antioxidant capacity, compared with the control group, the catalase activity in the serum of 1% treatment group decreased obviously at 14 days before delivery and 21 days after delivery(P<0.05); the malondialdehyde(MDA) content in the serum of 1% treatment group decreased significantly at 7 days before delivery and on the day of calving(P<0.01) and it decreased obviously on the 14th day after delivery(P<0.05); the MDA content in the serum of 2% treatment group decreased obviously at 7 days before delivery(P<0.05) and it decreased significantly at the day of calving and the 14th day after calving(P<0.01). Regarding to the reproductive hormone, compared with the control group, the estradiol(E2) content in the serum of the 2% experimental group decreased obviously on the 21th day before delivery(P<0.05), decreased significantly on the 14th day before delivery(P<0.01), and increased significantly on the 7th day before delivery(P<0.01). For the cow reproduction, compared with the control group, the incidence of endometritis and retained placenta in the 2% experimental group was lower, and there was no mastitis, and the first mating time were advanced 15 days. It can be seen that adding probiotics fermented Chinese herbal compound in the diet could significantly improve the intake of China Holstein, reduce the incidence of postpartum diseases, promote postpartum estrus of China Holstein, and advance the first breeding time. Adding 2% Chinese herbal compound to basal diet showed optimal effect.

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周花艳,杨泽曹,张虹亮,艾佳人,李志才,王水莲,王栋.发酵中草药复合物对奶牛抗氧化和繁殖性能的影响[J].湖南农业大学学报:自然科学版,2021,47(4):.

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  • 在线发布日期: 2021-08-11
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