Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS) was used to analyze the metabolites between Guidingxueya tea(NW34) containing higher epigallocatechin 7-O-gallate (EGCG) and the control group(NW48). XCMS software, METLIN database and SIMCA-P software were used to perform qualitative analysis, quantitative analysis and differential screening of metabolites. A total of 26 significantly different metabolites were identified, including 8 amino acids and peptides, 4 flavonoids, 4 organic acids, 3 nucleic acids and their derivatives, 2 alkaloids, 2 aldehydes, 1 sugar, 1 phenol, and 1 alkene. Pathway enrichment analysis showed that these differential metabolites are mainly distributed in 12 metabolic pathways, of which flavonoid biosynthesis contains the most differential metabolites(5 metabolites), followed by the degradation of aromatic compounds pathway(4 metabolites). In addition, the EGCG, epicatechin(EC), carbohydrates and amino acids metabolites contained in the NW34 strain are significantly higher than those in the NW48 strain, while the content of caffeine is lower than that in the NW48 strain, which indicates that the NW34 tea plant is more suitable for processing into green tea.