To investigate the molecular mechanism on cell division of Pasteurella multocida(Pm), different serotypes genomes of Pm from different hosts were downloaded from GenBank database and were subjected to extensive analysis. First, genes encoding Pm cell division-associated proteins in different strains were screened and then the operon with minC was identified by bioinformatics methods. The results showed that a total of 33 genes encoding Pm cell division-related proteins were found, of them 14 genes were existed in all studied Pm strains. Genes encoding the related proteins of the Noc system and minD and minE protein of minC system were not found in all Pm strains. However, a operon with minC, deoxyribonuclease denV and one unknown-functional gene was found. The results on protein interaction with deoxyribonuclease denV showed that some proteins interacting with denV shared domains related with cell division. The growth curves of porcine different serotypes Pm strains showed no difference among different strains, which Pm cell division had same mechanisms among them. It was suggested that Pm cell division involved by multi-proteins. The ftsZ polymerization mechanism in this process has specific mechanisms different from other bacteria and deoxyribonuclease denⅤ might involve the formation and localization of the Z ring of Pm cell division.