Physical and chemical properties, evolutionary relationship, protein structure and cis-acting elements of myelocytomatosis proteins(MYCs) gene family in tea plant were analyzed by bioinformatics methods, and qRT-PCR was used to study the expression patterns of MYC gene family in. The results showed that tea plant MYCs(CsMYC protein) were acidic, unstable, hydrophilic, non-secretory and non-transmembrane proteins. Subcellular localization predictions indicated that the 6 MYC genes were all in the nucleus. Gene structure and motif analysis showed that the secondary structures of MYC genes were mainly random coils and α-helix, and they all contained an exon and no introns. Moreover, members with similar motif distribution had similar tertiary structures. Evolutionary analysis showed that the CsMYC genes in tea plant were closer to AtMYC genes in Arabidopsis. Analysis of cis-acting elements suggested that the gene family mainly contained circadian, methyl jasmonate(MeJA) and abscisic acid (ABA). Results of qRT-PCR showed that the 6 CsMYC genes were mainly expressed in the roots and seeds, and might play important regulatory roles in the process of selenium absorption and accumulation in tea plant.