According to the sequence in GenBank, 3 pairs of specific primers were synthesized for duck circovirus (DuCV) REP gene, goose parvovirus (GPV) NS1 gene, duck enteritis virus (DEV) UL6 gene, and the virus clone plasmid was used as a template. The annealing temperature and final primer concentration were optimized to establish a triple PCR detection method that can simultaneously identify DuCV, GPV and DEV, and test the specificity, sensitivity and repeatability of the method. The results showed that the optimal conditions included the annealing temperature 58.5 ℃ and the optimal primer concentrations for DuCV REP, GPV NS1, DEV UL6 0.9, 0.6, 0.7 μmol/L, respectively. This detection method were sensitive to DuCV, GPV and DEV, but cannot amplify NDV, RA, AIV, DHV and TUMV, indicating that the method has strong specificity; the minimum detection volumes of this method for plasmid DuCV, GPV and DEV templates were 1, 100, 10 fg, indicating that the method is sensitive; using this method to detect DuCV+GPV+DEV, DuCV+GPV, GPV+DEV, DuCV+DEV, DuCV, GPV, DEV, amplification of specific fragments consistent with expectations indicates that the method has good reproducibility. 42 clinical samples were detected by this method, and the detection rates were 26.19%, 30.95%, and 19.05%, which were comparable to those of a single PCR, indicating that the method can be applied to the detection of clinical samples. The method established in this study has the characteristics of rapidness, simplicity, strong specificity, good sensitivity and good reproducibility. It can be used for the rapid diagnosis and differential diagnosis of mixed infection of DuCV, GPV and DEV clinical samples.