Cuurbita moschata was used as the materials. The content of polycyclic aromatic hydrocarbon in Cuurbita moschata roots, stems, leaves, flowers, fruits was determined by high-performance liquid chromatography to analyze the distribution, migration and accumulation of polycyclic aromatic hydrocarbon. The result showed that the male flowers and young leaves had only 3 species, while young fruits had 11 species. The average content of polycyclic aromatic hydrocarbon was 994.11 μg/kg in Cuurbita moschata organs, in which the average content of vegetative organs was 1169.59 μg/kg, and the average content of reproductive organs was 854.11 μg/kg. Chrysene content in organs of Cuurbita moschata was ranging from 607.28 to 953.75 μg/kg, which was higher than the content of the other 15 polycyclic aromatic hydrocarbons. Polycyclic aromatic hydrocarbon in organs of Cuurbita moschata were dominated by 2, 3 and 4 rings, of which the sum of the 4 rings was 7364.27 μg/kg, accounting for 81.18% of the total content of polycyclic aromatic hydrocarbon. The content of 5 and 6 rings was 339.27 μg/kg, accounting for only 3.74% of the total content of polycyclic aromatic hydrocarbon. Positive correlation was found between the total content of polycyclic aromatic hydrocarbon and water content in Cuurbita moschata.