In order to screen out optimal shade-tolerant soybean germplasm resources suitable for intercropping in mountainous environment, 30 soybean germplasm resources in Guizhou intercropped with maize-soybean were used in this study. The 10 agronomic characters and yield of the test materials under intercropping were collected and evaluated. The results showed that in the intercropping system, the yield of maize in each plot was not significantly different, while the yield loss of soybean was significantly lower than that of the net intercropping system. The inspection traits of the samples had some variation under intercropping conditions, and the variation coefficient is between 8.2% and 62.0%, indicating that the soybean germplasm resources have rich phenotype diversity. The grey correlation analysis showed that the traits affecting intercropping soybean yield were single grain number, effective pod number, stem length, weight of 100 grains, effective branch number, main stem node number, height of bottom pod, plant height and reflux rate. The 30 soybean materialscould be grouped into three categories: the shade resistant group, the intermediate group and the sensitive groups. These groups showed obvious difference on the studied traits. Among them, the group owning many single plants, many branches, and moderate weight, is the optimal soybean material suitable for intercropping in mountain environment.