In this study, three pairs of primers were designed based on the VP4 gene of giant panda rotavirus strain CH-1. A loop-mediated isothermal amplification(LAMP) detection technique of giant panda rotavirus was developed to optimize the reaction conditions and verify its specificity and sensitivity. The results showed that under the condition of 62 ℃, to join the final concentration of 8 mmol/L Mg2+, 0.6 mol/L betaine, 12 U DNA polymerase, ladder banding could be observed clearly after 40 min amplification. 1×LAMP visible light dye was added into the system to directly judge the amplification result by color change. When positive amplification occurred, the sample color changed from purple to blue. The LAMP detection was of high specificity and only amplified for giant panda rotavirus. The coefficient of variation within and between batches was less than 1%. With a sensitivity 100 times higher than that of conventional PCR and a minimum detection limit of 5×10-5 ng/μL RNA. The method was used to detect rotavirus in 50 samples of giant panda feces, and a total of 22 positive samples were detected, which was 100% consistent with the fluorescence quantitative PCR method. In summary, this method is simple to operate, short reaction time, strong specificity and fast result determination, and can be used for rapid detection of giant panda rotavirus in clinic.