The pot experiment was conducted to study the effects of applying Pterocypsela laciniata(P. laciniata) straw on growth and selenium accumulation of grape seedlings. The results showed that the application of the different parts of P. laciniata straw in the soil decreased the root, stem and leaf biomass of grape seedlings, and also decreased the photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoids) contents in grape seedlings. Among them, the application of leaf straw of P. laciniata decreased the root, stem and leaf biomass of grape seedlings by 29.76%, 22.50% and 22.33%, respectively, compared with the control. Application of the different parts of P. laciniata straw enhanced the activity of superoxide dismutase in grape seedlings, but had no significant effect on the content of malondialdehyde, and the stems and the leaves of P. laciniata straw applied alone reduced the activities of peroxidase and catalase. The different parts of P. laciniata straw application increased the selenium contents in the roots, stems and leaves of grape seedlings, and the leaf straw of P. laciniata application increased the selenium contents in the roots, stems and leaves of grape seedlings by 11.66%, 22.99% and 23.35%, respectively, compared with the control. Therefore, applying the different parts of P. laciniata straw in the soil could inhibit the growth of grape seedlings, but promote the absorption and accumulation of selenium in grape seedlings.