Ultra-high performance liquid chromatography (UPLC) method was used to determine the contents of 6 types of monomeric phenols(rutin hydrate, epicatechin, resveratrol, caffeic acid, gallic acid, catechin) from 6 Vitis vinifera × Vitis amurensis red wine grape varieties (strains) (‘Beihong’ ‘Beimei’ ‘Beixi’ ‘Beixin’ ‘Xinbeichun’ ‘Beichun’) during fruit ripening. Real-time fluorescent quantitative PCR was used to detect the expression of monomeric phenol synthesis related genes (PAL, C4H, F3'H, F3'5'H). The results showed that Beihong grape fruits contained high total phenols and tannins, which were 13.60 mg/g and 4.69 mg/g respectively; except for epicatechin, the content of the other 5 monomeric phenols in Beihong grape fruit is high, and the catechin content is the highest, which is 34.83 mg/kg. Relative expression levels of PAL and C4H genes in Beihong grapes are relatively high; except for catechin and gallic acid, caffeic acid and F3'5'H gene expression, epicatechin and F3'H gene expression, and rutin and C4H gene expression were significantly positively correlated in Beihong grape, with correlation coefficients both being 0.99. In Beixi grapes, rutin was significantly positively correlated with the relative expression of C4H gene, with a correlation coefficient of 0.99, and was extremely significantly positively correlated with F3'5'H gene expression, with a correlation coefficient of 1.00.