In this study, qRT-PCR was used to analyze the expression patterns of OsRZFP34 under various conditions including the high temperature (42 ℃), low temperature (4 ℃), simulated drought with 10% polyethylene glycol (PEG) 6000, salt (100 mmol/L NaCl) and ABA (100 μmol/L) treatments. The promoter of OsRZFP34 was cloned and its sequence was analyzed using online software New PLACE and PlantCARE; The OsRZFP34pro::GUS expression vector was constructed and transformed into rice; The activities of OsRZFP34 promoter driving GUS gene expression under different stress conditions were analyzed by histochemical assay and GUS enzyme detection. The results of qRT-PCR showed that the expression of OsRZFP34 could be induced by the above treatments. High temperature stress had the strongest induction on the expression of OsRZFP34, while ABA had only a weak induction on its expression. The results of the cis-regulatory element analysis show that, the promoter sequence contains a number of elements related to stress response, hormone response and Ca2+ signal transduction. The results of histochemical assay and quantitative GUS fluorescence analysis showed that the activity of OsRZFP34 promoter was regulated by high temperature, low temperature, PEG, salt and ABA treatments, which indicated that OsRZFP34 promoter was a multi-stress-inducible promoter.