In this experiment, the BL-420E biological function experiment system was used to investigate the effect of Gelsemium elegans Benth extraction by ethanol(GEBEE) on the independent contraction of isolated intestinal muscles in rabbits and the spasmodic contraction induced by Ach, BaCl2 and HA, and to explore the effect of sarcoplasmic reticulum Ryanodine receptor blocker RR, IP3 receptor blocker HP and nitric oxide synthase inhibitor L-NAME on GEBEE. The results showed that 0.16-0.64 mg/mL of GEBEE could inhibit the voluntary contraction frequency and tension of isolated rabbit intestinal muscles in a dose-dependent manner. The 0.32 mg/mL of GEBEE significantly inhibited the contraction frequency and tension of isolated rabbit intestinal muscles induced by BaCl2 and HA(P<0.05) and the contraction frequency of isolated rabbit intestinal muscles induced by Ach(P<0.05), while highly significantly inhibited the contraction tension of isolated rabbit intestinal muscles induced by Ach(P<0.01). For the contraction frequency and tension of isolated rabbit intestinal muscles pretreated with GEBEE, RR, HP and L-NAME showed no effect, significant enhancement and significant antagonism(P<0.05), respectively.