The effects of different concentrations (50, 100, 150 and 200 μmol/L) of melatonin soaking branches on the cadmium accumulation in the grape cuttings were studied by pot experiment. The results showed that melatonin soaking branches increased the biomass and cadmium content of grape cuttings under cadmium stress. With the increase of melatonin concentration, the root and shoot biomasses of grape cuttings increased first and then decreased, and the root and shoot biomasses with 150 μmol/L melatonin increased by 20.78% and 57.62%, respectively compared with the control. Cadmium content in roots and shoots of grape cuttings showed an increasing trend, and the cadmium content in roots and shoots with 200 μmol/L melatonin was increased by 559.53% and 113.27%, respectively, compared with the control. The melatonin soaking branches also enhanced the photosynthetic pigment content and antioxidant enzyme activity of grape seedlings. Therefore, the melatonin soaking branches promoted the growth of grapes, but also promoted the cadmium accumulation of grapes, thus could be not suitable for grape production in cadmium- contaminated areas.