In this study, we tested the acute toxicity in mice and the subchronic toxicity in rats of Nühuang granules based on the “guidelines for the acute toxicity test of veterinary drugs” and “guidelines for the 30-days and 90-days feeding tests of veterinary drugs” in the “Compilation of Technical Guidelines for Veterinary Drug Research”. The drug group was administered by intragastric administration with 16, 4, 1, 0.5 g/kg doses once and the status of the mice were observed for consecutive 7 days. The collected data was used to determine the median lethal dose(LD50). The drug group was administered with a dose of 90 g/kg to determine the maximum dosage with observation for consecutive 14 days. The drug group of rats was administered with 18, 6, 2 g/kg dose respectively once daily for 30 days. The clinical manifestations and their body weight were observed. At the end of the experiment, the blood physiology and biochemical indexes were measured, and histopathological changes were observed to determine its subchronic toxicity. The results showed that, during the acute toxicity test, no animal was dead, and thus LD50 was not able to be determined. During the maximum test period, the animal showed normal clinical performance, no animal was dead, and the maximum dosage of Nühuang granules was 90 g/kg. During the test period, the rats appeared normal mental state as well as the blood physiological and biochemical indexes of drinking water were within the normal range. The pathological examination revealed no lesions. The results revealed that Nühuang granules is non-toxic indicating that it might be of high drug safety.