In order to identify the inverted repeating PRE1 element in the 3'-untranslated region(3'UTR) of protein-coding gene porcine at whole genome-wide, the bioinformatics analysis of the repeating sequence elements was performed on the 22 342 protein-coding genes of the pig genome. The results showed that the short interspersed nuclear element(SINE) and the simple_repeat in the 3'UTR sequence of the pig protein-coding gene accounted for the highest proportion of the repeat sequence, which were 27.58% and 31.08%, respectively. Among the repetitive elements of SINE/tRNA, Pre0_SS and PRE1x components accounted for the highest proportion, which were 41.83% and 37.51%, respectively. After layer analysis, a total of 1 094 candidate protein-coding genes contained an IRPRE1 element in the 3'UTR. Gene Ontology analysis indicated that these candidate genes were mainly involved in mRNA splicing, via spliceosome, cell division, RNA splicing, via transesterification reactions, T cell activation, RNA splicing, T cell receptor signaling pathway, response to glycoside, triglyceride homeostasis, positive regulation of extrinsic apoptotic signaling pathway and cholesterol biosynthetic process. KEGG pathways showed that these candidate genes were mainly involved in Valine, leucine and isoleucine degradation, TNF signaling pathway, T cell receptor signaling pathway, RIG-I-like receptor signaling pathway, Phagosome, Spliceosome, Bile secretion, Thyroid hormone synthesis and Apoptosis. In addition, the IRPRE1 element in the 3'UTR of the three protein-coding genes was identified and was found to be widely expressed in multiple tissues.