The intestinal bacteria of larvae in Spodoptera frugiperda(S. frugiperda), that migrated in Changde area Hunan province, were isolated by traditional culture method and identified by 16S rDNA. In total, 11 strains of bacteria were obtained, belonging to genus of Enterobacter, Pantoea, Lelliottia, Bacillus and Klebsiella. Enterobacter, Bacillus and Klebsiella identified from S. frugiperda are similar to the same genus isolated from the gut of larvae in Mythimna separata. S. frugiperda could survive in Zea mays, Vigna unguiculate, Capsicum annuum and Brassica oleracea, and the larval survival rates of S. frugiperda are 70%, 50%, 17%, 10%, respectively. These plants could be the potential hosts of S. frugiperda in Hunan province.