PEP1 protein is one of the effector proteins of the Ustilago maydis. The study of PEP1 protein plays an important role for the new method development to combat the corn smut. In this study, the structure of pep1 gene was expressed, the resulting proteins were purified and analyzed by bioinformatics method. The recombinant expression plasmid pET-28a-pep1 was constructed, and Escherichia coli BL21 (DE3) was used as a host strain and induced with 1 mmol/L IPTG. The results of SDS-PAGE showed that the size of the induced expression product PEP1 was consistent with the theoretical value (20 800), indicating that pET-28a-pep1 is a capable strain to highly express the target protein in E. coli BL21 (DE3).