Using Changshun blue-eggshell chicken as the test object, single-nucleotide polymorphisms were screened in g.45670562-g.45670994 and g.45669431-g.45670163 of OC-116 gene by direct sequencing of PCR products; the genetic effect of polymorphic loci on shell quality of Changshun blue-eggshell chicken was analyzed using a general linear model. The results show that there are 4 SNP loci in the OC-116 gene, which are g.45668081 G>T on exon 2, g.45669143 T>C on exon 3, and intron 3 g.45669061 A>G and g.45669101 T>A. The g.45668081 G>T and g.45669143 T>C are synonymous mutations. The all four SNP loci show moderate polymorphism. The result of Chi square(χ2) test showed that the genotype distribution of the four SNP loci localized in the range of Hardy-Weinberg equilibrium(P>0.05). And, the correlation analysis showed that the g45669061 A>G loci had eggshells of AA and AG genotypes with the thickness significantly higher than that of GG genotype(P<0.05) and the g.45669143 T>C locus had eggshells of TT and CT genotypes owing significantly higher in egg shell strength and egg shell thickness than CC genotype(P<0.05). This results reveal the g.45669061 A>G and g.45669143 T>C sites can be used as marker sites for eggshell trait selection.