In this study, juvenile soft-shelled Pelodiscus sinensis with initial body weight(37.44±0.18) g were divided into four groups, each with three replicates, with 40 turtle in each replicate. The diets supplemented with four levels of Spirulina(0%, 5%, 8% and 10%) were fed for 12 weeks to evaluate the effects of Spirulina on growth performance, body composition, serum biochemical indexes of Pelodiscus sinensis. The results showed that survival rate, weight gain rate and specific growth rate were increased significantly in 8% group(P<0.05). Similarly, the crude lipid, total amino acid, delicious amino acid and essential amino acid in 8% group were higher than other groups with no significant variance in other groups(P>0.05). Crude protein was increased with the increasing dietary Spirulina content. In 5% group, content of TP and ALB were highest, the content of ALB was significantly higher than the control group(P<0.05). There were no significant differences in content of TG in four groups(P>0.05), but significantly decreased the ALT activity(P<0.05), as the supplement increased, the T-CHO content gradually decreased. The results demonstrated that dietary supplementation with Spirulina could be helpful for promoting weight gain rate, survival rate and specific growth rate and for improving nutritional level and flavor. When the additional was 8%, the effect of promoting growth was the most significant, and the content of amino acid in muscle was the highest. Adding 8% Spirulina to feed can increase the content of crude protein and crude lipid, reduced the ash content, which was beneficial to protein deposition and improved quality of Chinese soft-shelled turtle. With 5% Spirulina, the TP and ALB content were highest, while the ALT activity lowest in all groups, which improved liver function of Pelodiscus sinensis. Our study concluded that the appropriate added content should be 5%~8%.