In order to reveal the cadmium enrichment in different organs and tissues of Pelodiscus sinensis, three concentration gradients of cadmium(5, 50, 100 mg/kg) were applied to the breeding soil separately and lasted for 75 d. The results showed that the group with 100 mg/kg cadmium owned significant differences from the control group (no cadmium applied) and the group with 5 mg/kg cadmium in aquaculture water, soil and different Pelodiscus sinensis organs and tissues and displayed “dose-effect” relationship. The sequence of cadmium concentration from high to low among the organs and tissues was liver, intestines, calipash and muscle. Both of the cadmium content in edible muscle and calipash in every group were lower than the national food limit hygienic standards. The results of correlation analysis showed that all three experimental groups in the first 30 days displayed significant positive correlation, and existed “time-effect” relationships, however the correlation between the aquaculture environment and the cadmium content in measured organs and tissues was not significant and even showed a negative correlation between sediment, calipash and liver in the control group, between water and liver, intestines in the group with 50 mg/kg cadmium, between water and liver in the group with 100 mg/kg cadmium. As the concentration was diluted and transformed in the water environment and metabolized by organs and tissues, there is no significant correlation between water, intestines and calipash, muscle on the 45th day; between intestines and the environment factors, the others organs and tissues and between muscle and water, calipash, liver on the 60th day; between water, calipash and intestines, liver and between intestines and sediment, muscle on the 75th day.