In this study, 36 samples of Miscanthus spp. including four species (M.sinensis, M.floridulus, M.sacchariflorus, M.lutarioriparius) from East China region were used to study the genetic diversity of the phenotypic characters of Miscanthus spp. during their overlapping region. Their 8 phenotypes were subject to multivariate variance analysis, correlation analysis, clustering analysis. The results showed the interspecific difference trend in the studied phenotypes of Miscanthus spp. decreases as the following order, tiller number, basal diameter, dry matter yield, inflorescence length, plant height, maximum leaf length, maximum leaf width, ratio of leaf length to width. The maximum leaf width and ratio of leaf length to width showed significant interspecific difference with P＜0.05 and the other six phenotypic characters showed significant difference within the species P＜0.01. And, there were significant interaction effects of eight phenotypic traits among and within species. The variation coefficient of all eight phenotypic traits was in the range from 7.94% to76.80%, and the Shannon’s index of all eight phenotypic traits was ranged from 1.27 to 1.78. The variation coefficient trend of four Miscanthus species was as follows: M.sinensis(26.16%), M.lutarioriparius(25.46%), M.sacchariflorus(24.49%), M.floridulus (17.17%). The Shannon’s index trend of the four Miscanthus species was M.sinensis (1.74), M.lutarioriparius (1.70), M.floridulus (1.68), M.sacchariflorus (1.60). The 36 Miscanthus germplasms could be clustered into three groups based on the euclidean distance at 20: all M.sacchariflorus species, in Ⅰ class, all M.sinensis and M.floridulus species in Ⅱ class, and all M.lutarioriparius species in Ⅲ class. The plant heigh of M.sinensis and latitude were significantly negatively correlated, the dry matter yield of M.sinensis was significant positively correlated with the altitude. The dry matter yield, tiller number and base diameter of M.sacchariflorus were negatively correlated with the latitude, while the yield factor phenotype of M.floridulus and M.lutarioriparius were not significantly correlated with geographical factors.