480 Pelodiscus sinensis(Chinese softshell turtles) were divided into 4 groups with 3 repeats in each group. These groups were then fed with a trial feed including no additions(CK), 50, 100 and 150 mg/kg sanguinarine respectively for 75 d in a bid to investigate the impacts of sanguinarine on their growth, body compositions and serum indexes. The results showed that the survival rates of the 50, 100 mg/kg groups were significantly higher than that of the 150 mg/kg group and CK. And the feed coefficients of the 50, 100 mg/kg groups were significantly lower than that of CK. The total amount the umami amino acids in turtle’s muscles in the 50 mg/kg group increased 1.7 mg/kg in contrast to CK. Furthermore, with the increase of the addition of sanguinarine in the feed, the ratio of crude protein in the body composition of Pelodiscus sinensis tended to increase first and then decrease. Specifically, the ratio of crude protein of the 50 mg/kg group was significantly higher than that of that of 150 mg/kg group and CK. Meanwhile, the glucose content in the serum of Pelodiscus sinensis tended to increase first and then decrease with the addition of sanguinarine. The glucose contents in the 50, 100 mg/kg groups were significantly lower than that of the 150 mg/kg group and CK. Moreover, the activity of alkaline phosphatase in the 50 mg/kg group was significantly higher than that of CK, and the activity of glutathione peroxidase in the 50 mg/kg group was significantly higher than that of the rest three groups. The activity of aspartate aminotransferase in the 50, 100 mg/kg groups were significantly lower than that of CK. In summary, under the condition of this study, it suggests that it would be more suitable to add 50-100 mg/kg sanguinarine in the basic feed of Pelodiscus sinensis.