The 154 pet-derived Staphylococcus(75 strains collected in winter and 79 strains in spring) isolated from pet hospitals in Urumqi were identified by agar dilution method to have 12 antimicrobial resistance(amikacin, clindamycin, gentamicin sulfate, florfenicol, tetracycline, rifampicin, enrofloxacin, penicillin, oxacillin, amoxicillin/clavulanic acid, ceftiofur, levofloxacin). The results showed that the resistance rates of winter pet source to oxacillin and penicillin were higher than 90.0%, the resistance rate to ceftiofur and tetracycline were higher than 50.0%. The resistance rates of levofloxacin in those collected from spring pets was up to 92.4%, the resistance rates to ceftiofur and tetracycline were above 80.0%. The chi square test results showed that resistance rate of Staphylococcus to oxacillin from winter pets was significantly higher than that from spring pet source(P<0.05). The resistance rates of Staphylococcus to florfenicol, rifampicin, levofloxacin, enrofloxacin and amoxicillin/clavulanic acid from spring pet source were significantly higher than that from winter pet source(P<0.05). The multidrug resistance results of winter pet source Staphylococcus was concentrated in 1-3, the multidrug resistance results of spring pet source Staphylococcus was concentrated in 4 resistant, 6 resistant and 9 resistant. The gene detection results showed that the main genes of winter pet source Staphylococcus were ermB(40.0%, 30/75) and fexA(22.7%, 17/75). The main detection genes of spring pet source Staphylococcus were fexA(39.3%, 31/79) and femA(20.3%, 16/79), cfr, fexB and tetA genes weren’t detected.