To characterize the biological function of AvrPii involved in rice PAMPs-triggered immunity(PTI) immune signaling, the properties of AvrPii protein encoded by AvrPii were analyzed, and the transgenic rice with AvrPii gene was constructed. The results showed that AvrPii protein is composed of 70 amino acids with a molecular weight of 7 500, the theoretical isoelectric point is 6.0 and the average value of hydrophilic amino acids is –0.159. The AvrPii protein includes one signal peptide, one transmembrane domain, and two conserved motif with residues LxAR and CxxCxxxxxxxxxxxH. Thirteen transgenic lines with full length AvrPii gene or AvrPiinsp gene without the signal sequence were obtained. PCR detection and expression analysis confirmed the insertion and the transcriptional expression of AvrPii and AvrPiinsp in the receptor cultivar Nipponbare.