A PCR-based co-dominant InDel marker, T9E4, was developed according to sequence information of the broad-spectrum and durable blast resistance gene Pigm, and was employed in molecular marker-assisted selection breeding for blast resistance improvement of the rice variety Xiangwanxian 13. Resistance responses to 21 tested Magnaporthe oryzae isolates were quite different between the Pigm donor Gumei 4 and receptor Xiangwanxian 13, presenting 95.2% and 61.9% resistance frequency respectively in Greenhouse inoculation assay. Gumei 4 showed high-level seedling and panicle blast resistance but Xiangwanxian 13 was highly susceptible in field nursery. The T9E4 marker primers amplified a nearly 750 bp band from Gumei 4 genome while the PCR product is about 1 800 bp from the receptor DNA template, which can be conveniently detected by agarose gel electrophoresis, and the marker-assisted selection efficiency reached 100% in this study. An elite BC5F3 line, harboring Pigm homozygous allele, possessing high-level resistance to seedling and panicle blast, and with similar agronomic traits to the receptor, was bred by consecutive backcross, self-fertilization and molecular selection.