To study the molecular mechanism of glucose metabolism in the slow muscle and the fast muscle of Siniperca chuatsi, the expression of 16 genes associated with glucose metabolism were measured in slow muscle and fast muscle by q-PCR. It showed that 4 genes related to glucose uptake, 4 genes related to phosphorylation pathway and 2 genes related to glycogen metabolism were significantly differently expressed in the slow and fast muscle, respectively. The expression of PI3K, MAPK5, MAPK6 were higher in the fast muscle comparing to the slow muscle, which indicated the uptake and utilization of glucose linking to the 3 genes were more important in fast muscle than slow muscle. On the contrary, the expression of MEF2α, PGC-1α, PGC-1β, PDK1, MAPK4, GYS1, GSK3β were higher in the slow muscle comparing to the fast muscle, which indicated the uptake and utilization of glucose linking to the 7 genes were more important in fast muscle than slow muscle.