In order to cultivate sheep strains of highly developed muscles, we cultured and transplanted reconstructed embryos, which were generated by somatic nuclear transfer with the nuclear donor cells from the frozen transgenic cell lines transfected with the RNA interference sequence of sheep myostatin gene(MSTN) from our previous work. The results showed that the cleavage rates of reconstructed embryos with the nuclear donor from fibroblasts and transgenic cell lines were 44.6% and 33.0% respectively. And, the morula proportion were 24.1% and 15.6% respectively. There was no significant difference between them. In addition, we experienced 1 fetal abortion after transgenic embryos transplantation of 23 recipients. The MSTN RNA interference sequence was integrated into the aborted fetus genome by use of PCR. This work provided evidence on obtaining the transgenic sheep with highly developed muscles through the RNA interference of MSNT gene.