To understand the effects of acrylamide water protectant on Kiyomi tangor, we treated Kiyomi tangor at the phase of flowering, fruiting, expansion and color transformation with five different mass contents of CLP: 60, 80, 100, 120 and 140 g of each plant, with naming T1, T2, T3, T4 and T5 respectively. Set a CLP–free treatment as the control (CK). The indexes of rhizosphere soil environment and fruit quality were measured and analyzed. The results showed that, with the increase of CLP usage, all phase of soil water content and soil enzyme activity except fruit expansion stage, soil respiration intensity except the fruiting stage, the number of actinomyces in flowering stage, the number of bacteria in the fruit expansion stage, the number of soil microorganisms, root activity and soil organic matter content all showed a increasing trendency first and then falling. The T3 treatment had the summit. Compared with the control group, CLP treatments significantly increased the content of vitamin C except T1, the reduced sugar and the total sugar content, sugar acid ratio, soluble solids content and single fruit quality, and all these indexes reached the highest level in T3 treatment, with lowest of the titrate acid content. Through comprehensive analysis, CLP can significantly improve the rhizosphere environment of soil and the quality of fruit, and the peformance of T3 treatment is the best.