9个白三叶品种对渗透胁迫和干旱胁迫的响应
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农业部财政专项(131621301352025)


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    摘要:

    以9个白三叶品种(海发、惠亚、超级惠亚、碧胜、G18、鄂牧1号、克塞、皮陶、克劳)为试材,分别进行10%聚乙二醇6000(PEG 6000)渗透胁迫种子发芽试验和苗期盆栽干旱–复水试验,通过加权隶属函数法对白三叶品种耐旱性进行评价。结果表明:萌发期PEG胁迫抑制了种子萌发,但各品种不同的萌发指标对PEG敏感程度存在差异,其中活力指数和发芽指数受抑制较为明显,发芽势和发芽率受抑制较轻微;萌发期耐旱性评价时权重较高的指标是相对根系体积、相对根系表面积和相对活力指数;苗期各品种对干旱的反应差异较大,大部分品种的根系生长因干旱胁迫而受抑制;苗期权重较高的指标是存活率、相对根系体积、相对地上生物干质量。各品种萌发期耐旱性由强到弱依次是海发、惠亚、超级惠亚、碧胜、G18、鄂牧1号、克塞、皮陶、克劳;苗期耐旱性由强到弱依次是克塞、超级惠亚、克劳、惠亚、鄂牧1号、碧胜、海发、G18、皮陶;萌发期和苗期耐旱性排序间没有相关性。超级惠亚的综合耐旱性最好。

    Abstract:

    Nine white clover varieties (Haifa, Huia, Super Huia, Zapican, G18, Emu No.1, Kersey, Pitau and Crau) were selected as sample materials, and the test of seed germination under 10% PEG 6000 osmotic stress and the pot incubation test of water stress and rewatering were detected respectively. The drought tolerance was evaluated by weighted fuzzy membership functions. The results showed seed germination were inhibited in PEG 6000 osmotic stress, but the tolerance of these germination indices to PEG 6000 stress is different, in which vigor index and germination index were inhibited significantly, germination potential and germination rate were inhibited slightly. The indexes of higher weight in the evaluation of PEG stress at the germination stage were relative root volume, relative root surface area, and relative vigor index. The responses of seedlings in different varieties to drought stress varied greatly. Root growth of most white clover varieties were inhibited by drought stress. The indexes of higher weight at the seedling stage were the survival rate, relative root volume, and relative dry weight of aboveground biomass. The order of drought tolerance of 9 white clover varieties at the germination stage was Haifa, Huia, Super Huia, Zapican, G18, Emu No.1, Kersey, Pitau and Crau. The order of drought tolerance at the seedling stage was Kersey, Super Huia, Crau, Huia, Emu No.1, Zapican, Haifa, G18 and Pitau. There was no correlation between the orders of drought tolerance at the germination stage and at the seedling stage. Super Huia had the best drought resistance.

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武建新,李丹竹,陈桂华,张鹤山,张志飞.9个白三叶品种对渗透胁迫和干旱胁迫的响应[J].湖南农业大学学报:自然科学版,2018,44(5):.

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  • 在线发布日期: 2018-10-23
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