T9501, a sterile mutant of sweet maize, was obtained from a field breeding in Hainan in 2014. It was subjected to mutant hybridization with B73 as the father, resulting in F1 in 2016. The F1 self–crossed and generate F2 in 2017. Using F2 group and its parental origin as testing materials, the pollen fertility identification showed that the pollen was little and sterile. Their genetic analysis revealed that the infertility traits were controlled by a pair of recessive genes. Polymorphism and linkage analysis of F2 and its parental origin using 128 pairs of insertion–deletion markers indicated that the target gene was located in the sterile gene preliminary maize chromosome 6 short arm (6.01 bin), named MS9501 in this study. Using a encryption labeling method developed, we determined the target genes was located in marking Indel130 between SNP2. The relative genetic recombination rate distance were 0.91 cM and 8.10 cM, with physical distance about 6.51 Mb.