A total of 120 mice were randomly divided into the control group, CC14 group, Dimethyl dicarboxylate biphenyl(DDB) group, low, medium, and high doses(1.0, 2.0, and 4.0 g/kg) of Terminalia chebua, Euphorbia fischeriana Steud per Terminalia chebula and Rubiacordifolia L. per Terminalia chebula groups(n=10). After drug delivery for 7 d，all mice were intraperitoneally injected with 10 mL/kg CCl4 to induce the liver injury. And then the activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) of serum and totasuperoxide dismutase(SOD), glutathion peroxidase(GSH–Px) in liver homogenates were examined. And the histological structure of the liver was observed. The results indicated that Terminaliachebula Retz. could significantly reduce the ALT and AST activities and enhance SOD, GSH–Px activities in the liver tissue and play a role in protection of the liver. The efficacy of these observation on Rubia cordifolia L. per Terminalia chebula were strongner than Euphorbia fischeriana Steud per Terminalia chebula, which was stronger than Terminalia chebula. And, high dose of these Terminalia chebula had better efficacies than that in low dose. Terminaliachebula Retz. has the protective effect on acute chemical liver injury induced by CC14, with the best effect at the doseage of 4.0 g/kg using Rubiacordifolia L. per Terminalia chebula. Its mechanism may be linked to the anti–oxidation induced by the Terminaliachebula Retz in liver.