In order to obtain mechanical property parameters of Longan branch, compressing, tensile and shear test of Longan branch during pruning period were conducted in the accurate electronic universal testing machine. The results showed that compressive strength parallel to grain of Longan branch ranged from 18.33 to 27.77 MPa and mean value was 23.68 MPa, and compressive modulus of elasticity ranged from 351.51 to 835.85 MPa and average value was 547.20 MPa. It was showed that an exponential function positive correlation between the stress resistance intensity and compression energy. Tensile strength parallel to the grain of Longan branch was in the range of 40.26–70.28 MPa and average value was 52.12 MPa. Shear strength of Longan branch ranged from 9.05 to 13.42 MPa and mean value was 11.44 MPa. There was a significantly positive linear correlation between maximum shearing force and the cross–sectional area, and significantly positive power function correlation between shear energy and cross–sectional area.