The 2 sites of SNP in Pelodiscus sinensis were verified by adopting tetra–primer amplification refractory mutation system PCR (ARMS–PCR) method, genotype frequency were counted as well. The relationship between six growth traits (body weight, carapace length, carapace width, shell length, body height and calipash width) and 2 SNP sites were analyzed by mean of one–way ANOVA. The result showed that there was only one significant difference in body height and calipash width on the site of A–1609G. The different genotypes on 2 SNP sites composed 6 different diplotypes (excepted for D1, D6 and D9, which frequency lower than 3%), and they were significant difference on all growth traits by the analysis of one–way ANOVA. The D4 belonged to dominant genotypes with the largest mean value on all aspects of growth traits. The result indicated that the 2 SNP sites of IGF2 gene could be used as a molecular marker–assistant breeding for growth traits of Pelodiscus sinensis.