The resistance of 59 new maize hybrid combinations against Fusarium graminearum stalk rot was determined by field artificial soil embedding method. The results showed that among the 59 maize combinations, 11 combinations showed high resistance (HR), 23 combinations showed resistance (R), 18 combinations showed middle resistance (MR)；1 combination was susceptible (S), 6 combinations were high susceptible (HS). The spike weight, grain weight and seed yield were reduced in the 59 maize combinations inoculated with Fusarium graminearum compared to those un–inoculated. The resistance combination (245 × 249) had higher value considering the disease resistance and spike and grain weight.