The inhibitory effects of Lonicera japonica, Plantago depressa and other 16 herbal extracts and nano–zinc, nano–silver on Vibrio harveyi (TCBS–G) from Fenneropenaeus chinensis were studied by means of disc agar diffusion and micro double dilution methods. The results showed that nano–zinc had a strong inhibitory effect on TCBS–G with the diameter of 35 mm inhibition zone, followed by Herba euphorbiae with the diameter of 25 mm. The MIC of nano–zinc and Herba euphorbiae detected on 96 well plates via multiple dilution method was 12.5 mg/mL, the value was 6.25 mg/mL for that of nano–silver. From the test of different inhibitors on biofilm formation of Vibrio harveyi by mean of MTT assay, we found that the inhibitory effect of nano–zinc on the formation of V. harveyi biofilm was significant, especially when the solution concentration was above 3.125 mg/mL (P<0.05), and there were also significant effects for Astragalus membranaceus and Herba euphorbiae, when their concentration were above 12.5 mg/mL (P<0.05). The result from susceptibility test of general drugs showed that the TCBS–G was very sensitive to Ceftazidime, Gentamicin, compound Sulfamethoxazole with less than 20 mm diameter of inhibition zone, however, it was not sensitive to Cephalosporins, Tetracycline etc.