Test materials of dominant blueberry cultivars, O'Neal, Misty, Brigitta, Sharpblue and Legacy from Chengdu, were selected to analyze their fruit morphological characteristics, flavor quality and bioactive substances. The results showed that the average fruit weight of the selected blueberries was 1.91 g; the fruit shape was dominated by oblate spheroid, and the fruit shape index ranged from 0.73 to 0.85; calyx was mainly close type, and their segment number ranged from 5–7. Dark blue was the dominant fruit color. The average content of soluble solids, reducing sugar and titratable acid were 12.26%, 9.14% and 1.22%, respectively; it showed an excellent flavor judged from the ratio of sugar and acid, which was 9.35. The average content of total active polyphenol, tannin, flavonoids and ellagic acid were 4.624, 0.346, 0.402 and 1.254 mg/g, respectively. Fruit quality and contents of bioactive substances kept stable among all cultivars, and most of indicators among all cultivars showed no statistical significance in the production area. The five cultivars were all suitable for planting in Chengdu, among of them, however, the optimal cultivars suitable for planting were Sharpblue and O'Neal.