The pepper cultivar Xingshuzhoupi was used as experiments materials, the overwintering pepper in plastic greenhouse was pruned on February 3 and 24, the indexes of phenological phase, yield and its components, fruit-related traits and the diseases and insect pests occurrence were measured to compare with the spring cropping planting pepper plants which were transplanted the same as the pruning time of the overwintering pepper. The results showed that: compared with the spring cropping planting pepper transplanted on the same day, the overwintering regenerated pepper plants reached the budding period, the initial flowering period and the initial harvesting date earlier, and had highly significant higher of the fruit number per plant, yield per plant and yield at the early harvest stage; the yields of the regenerated plants pruned on February 3 and 24 were 32.36% and 45.40% higher than those of the spring cropping planting pepper transplanted on the same day respectively; the fruits, including fruit weight, longitudinal diameter and transverse diameter of the overwintering regenerated pepper plants were significantly smaller than those of the spring cropping planting pepper; the flesh thickness of the spring cropping planting pepper plants were highly significantly thicker than that of the overwintering regenerated pepper plants; the diseases and insect pests occurrence, such as the epidemic disease, whitefly and red spider mite of the overwintering regenerated plants were more serious than those of the spring cropping planting pepper.