To analyze the characteristics of insulin–like growth factor–binding protein 1(IGFBP1), and to reveal its expression pattern in different gender tissues and the expression difference of its protein between male and female muscles, the IGFBP1 cDNA was cloned from the Pelteobagrus fulvidraco. The mRNA expression of IGFBP1 was studied using florescent quantitative real–time PCR and the protein expression pattern of IGFBP1 was detected using western blot method. The full–length of cDNA of IGFBP1 is 1 259 bp which included a 138 bp complete 5' untranslated region (5′ UTR), a 407 bp 3′ UTR and a 714 bp open reading frame (ORF) region which encoded a 237 amino acid of peptides. The results indicated that nucleotide sequences of IGFBP1 were obvious difference from other species (such as Hypophthalmichthys molitrix, Cyprinus carpio, Carassius auratus, Ctenopharynodon idellus and Megalobrama amblycephala, etc.) except for Ictalurus punctatus, which was identical to Siluriformes of Pelteobagrus fulvidraco. The highest abundance expression of IGFBP1 transcript was detected in liver, and mRNA level of IGFBP1 in heart, kidney, muscle and intestine from male was higher than that from female (P<0.05). The IGFBP1 protein expression from male was higher than that from female. The results provided data support for further functional investigation on IGFBP1 gene in Pelteobagrus fulvidraco.