Maise seedlings from 22 of maise varieties were inoculated with Ustilago maydis by artificial injection, and disease resistance of these maise varieties were evaluated by onset of symptom, incidence rate and disease index. The results showed that the infection symptom was first observed at eight days post inoculation (dpi), the diseased maize of all varieties developed quickly since 13 dpi and become outbreak until 16 dpi and disease status become stable from 18 dpi. Disease resistance was different among maize varieties: varieties LD901 and TPY891 showed to be high resistant, varieties JK665 and JN628 showed to be middle resistant and 12 varieties of maize, such as NS1, JD38 and NM1 showed resistant to Ustilago maydis while varieties N2000, TPY98, Golden Bautam, ZCTN8, 15H–09 and LSXF F1 showed to be susceptible to Ustilago maydis.