The study was designed to identify and construct a library for swine phage single-chain antibody (scFv) rSpaA. Total RNA was extracted from splenic tissue and used to amplify fragments consisting of a variable heavy chain (VH) and a variable light chain (VL). After purification, VH and VL were joined with a linker to produce scFv fragments by splicing-overlap-extension (SOE) PCR. The gene scFv was cloned in plasmid pComb3Xss, and the recombinant phagemids were transformed to susceptible E. coli XL1–Blue. After infection with the aid of phage M13K07, the library of phage antibody was constructed. The size of constructed antibody library was 2.5×106. By taking the purified recombinant protein rSpaA as target antigens, 8 strains of positive scFvs with binding affinity to rSpaA were identified after 5 rounds of procedure of ELISA: bind-elution-enrichment. In conclusion, the construction of rSpaA swine scFv library and the study for the preparation of recombinant antibodies could provide a new way and lay the foundation for the immunoassay of SE and comprehensive prevention and control to swine erysipelas.