蛹虫草中蓝光受体基因Cmwc–1和Cmwc–2的表达特性分析
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国家自然科学基金项目(31060004;31260010)


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    摘要:

    采用 RT–PCR克隆蛹虫草中蓝光受体基因Cmwc–1和Cmwc–2。结果表明,Cmwc–2基因序列的翻译产物CmWC–2中存在1个PAS结构域和1个锌指结构域。实时荧光定量PCR结果显示,蛹虫草菌丝体Cmwc–1和Cmwc–2基因的表达量分别在蓝光照射后6 h和2 h达到峰值。子实体形成不同阶段Cmwc–1和Cmwc–2的相对转录量都有较大差异,生长50 d时蛹虫草子实体不同部位的Cmwc–1表达主要在子实体的中下段,Cmwc–2的表达主要在子实体的顶端和中段,畸形子实体中Cmwc–1和Cmwc–2的表达量都不高。

    Abstract:

    ORF sequence of gene Cmwc–1 and Cmwc–2, the blue light receptor, were cloned from fungus Cordyceps militaris by approach of RT–PCR. There were 1 PAS functional domains and 1 zinc finger functional domain at putative CmWC–2 amino acid sequence in Cmwc–2 ORF. The real-time PCR results showed that the translation of gene Cmwc–1 and Cmwc–2 reached their peaks in 6 h and 2 h, respectively. The relative transcription level of gene Cmwc–1 and Cmwc–2 were quite different according to their growing stages. The relative transcription level of Cmwc–1 was mainly distributed at the middle and lower section of fruiting body, and rarely spotted at the top after 50 d inoculation, while, it was mainly at the top and the middle section for Cmwc–2. There were little relative transcription level of Cmwc–1 and Cmwc–2 in abnormal fruiting body.

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李琴,毛玉梅?,付鸣佳,沈俊良,金华燕,钟雪晴.蛹虫草中蓝光受体基因Cmwc–1和Cmwc–2的表达特性分析[J].湖南农业大学学报:自然科学版,2016,42(6):.

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  • 在线发布日期: 2016-12-02
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