Different concentration of biogas slurry (1∶4, 1∶6, 1∶8, and CK) and different diameters of soil hole condition(3, 5, 7 cm) were designed in the laboratory to investigate the characteristics of interference infiltration in soil under hole irrigation with biogas slurry. The results showed that the cumulative infiltration decreased with the concentration of biogas slurry increase during the same infiltration time, while they increased with diameters of soil hole at the same biogas slurry concentration; soil water infiltration rate decreased with the infiltration distance increase at the same biogas slurry level. The higher the concentration of biogas slurry, the lower the infiltration rate at the same diameter and infiltration distance. Infiltration rate increased gradually with the diameter of soil hole at the same infiltration sites. The case that the cumulative infiltration increased with time could be better described by Kostiakov infiltration model. The correlation coefficient (R2) from all treatments were larger than 0.99. The areas that soil water content larger than or equal to 22% increased with the diameters of soil hole, and they also increased with the biogas slurry decrease. These results demonstrated that the interference infiltration rate, cumulative infiltration and soil water content were jointly affected by biogas slurry concentration and diameter of soil hole.