The study aims to clone the pig insulin induced gene 1(INSIG1) and conducts its protein sequences analysis. According to conserved sequence gene INSIG1 in human, a pair of primers was designed. By adopting pig total RNA as template, the INSIG1 gene sequences was cloned using RT–PCR, then the INSIG1 gene was connected to pMD 19–T Simple vector. The positive clone was sequenced after the identification of clone by PCR, and the sequence characterization was performed from biology information analysis software. The results from relative real-time PCR analysis indicated that INSIG1 transcript abundance was the highest in lung, next in liver, spleen, and heart in turn. The experiment obtained a 999 bp sequence, of which included 831 bp open reading frame encoded with 276 amino acids. From the results of bioinformatics analysis showed that the protein does not contain signal peptide sequence, and from the results of amino acid sequence alignment showed that pig INSIG1 had high similarity score (more than 71%) with other animals in GenBank.