Pteris vittata was extracted with percolation method by methanol, composition of the extract was identified by GC-MS, antioxidant activities of the methanol extract were evaluated by six common tests and Trolox was used as positive control. The results showed that: the highest content of methanol extract was esters, account for 54.22%, alcohol was the second, account for 24.03%, and ketones, acids and indoles matter were 10.44%, 6.73% and 1.34% respectively. The flavonoids content of extract was 4.81%. DPPH scavenging activity was stronger at the six tests compare with Trolox, and the EC50 value was 0.15 mg/mL, the EC50 of lipid oxidation inhibition, superoxide, hydroxyl radicals and hydrogen peroxide scavenging activity were 6.55, 0.09, 4.11, 0.45 mg/mL respectively.