In order to investigate the effects of glue fibrin (FG) on the transplantation of autologous mesh skin graft in dogs, 15 dogs as experimental animal were selected to set up a model of whole layer skin defection with 3 cm×4 cm at their two forelimbs. The chest and back were selected as donor area; By adopting the usage of FG in dog mesh skin graft surgery as test group and the single mesh skin graft surgery as control, three dogs were randomly selected for whole and part observation in skin graft at the 3rd, the 5th, the 7th, the 14th and the 28th day, the paraffin sections were made for histological observation as well. The part observation result showed that swelling and exudation in the test group was lighter than that in the control, inflammatory reaction period was shorter in the test group; after surgery of 28 days, all skin grafts grew well, and linear scar in the test group was less than that in the control. The result from histopathology observation showed that the time of inflammatory cell infiltration was short; the collagen fibers arranged regularly; and skin appendages was complete in the test group. The results showed that FG could shorten the inflammatory response period, enhance the ability of local tissue repair and reduce scar hyperplasia, which made the structure and function of survival skin grafts closer to the normal skin.