Genome segments S8 (encoding minor core capsid protein), S9 (encoding virplasm and non-structual protein) and their chimeric sequences S8&S9 of RBSDV were used to construct six RNAi vectors and transferred into rice cultivar Wulingjing 1 to generate transgenic plants by Agrobacterium-mediated transformation. Transgenic plants were first screened by immersion with hygromycin solution to eliminate the false-positive plants, then identified by PCR genotyping analysis from the DNA level，followed by qRT–PCR analysis of candidate genes at the RNA level. Single-copy transgenic lines with high expression of S8 detected by qRT–PCR were picked up for Laodelphax striatellus-mediated virus transmission test, and the results showed that transgenic lines containing S8 RNAi vector displayed resistance to RBSDV.