In this experiment, tetraploid Dendranthemaindicum var. aromaticum and Dendranthema × gandiflorum ‘niu9717’ were used as material to study pollen and stigma morphology, pollen viability and stigma receptivity, the optimal pollen germination medium, and analyse seed setting rate in different hybrid method and morphological characteristics of F1 plants. The results showed that both tetraploid Dendranthemaindicum var. aromaticum and Dendranthema × gandiflorum‘niu9717’ had single pollen grain with three germination ditch and stigma pollinating in the bifurcation stage. The best medium for pollen germination was ME3 + 150 g/L PEG 4000 + 150 g/L Sucrose + 0.1 g/L H3BO4 + 0.1 g/L Ca2+. Tetraploid Dendranthema indicum var. aromaticum had the highest pollen viability on the fourth day of blossom (64.63%) and at 13:00 pm in a day (54.56%) and the strongest stigma receptivity on the 9th day of blossom and at 13:00 to 14:00 in a day, while Dendranthema × gandiflorum ‘niu9717’ had the highest pollen viability on the 6th day of blossom (11.63%) and at 13:00 pm (9.26%) in a day and the strongest stigma receptivity on the 4th to 5th day of blossom and at 12:00 to 13:00 in one day. Comparing with different pollination ways of tetraploid Dendranthema indicum var. aromaticum and Dendranthema × gandiflorum‘niu9717’, the way of emasculation, bagging and hand cross-pollination had the highest seed setting rate ( 79.28%) and obtained 21 F1 hybrids with different characteristics in plant morphology, leaf and flower bud, which 2 plants with creeping growth, 8 plants with leaves of length≤width, 7 plants’ with leaves of blunt sawtooth margin , 10 plants with earlier flower budding , and most of the buds were dark brown, only 1 was green.