An inbred glutinous rice of Yuxiangnuo 1 was used as experimental material, three nitrogen rates (N0, 0 kg/hm2; N120, 120 kg/hm2; N180, 180 kg/hm2) and two nitrogen application patterns (pattern A: 70% was applied as basal fertilizer and 30% in early tillering stage; pattern B: 50% N as basal fertilizer, 20% and 30% in early tillering and panicle initiation stage respectively) were designed to study the effect of nitrogen rates and nitrogen application patterns on nutrient accumulation and nitrogen use efficiency of glutinous rice. The results showed that: There was significantly difference in uptakes of nitrogen (N), phosphorus (P) and potassium (K) of glutinous rice among different nitrogen rate treatments. The uptakes of N, P, K of pattern B were less than those of pattern A. The demands of N, P, K per 1 000 kg grains of pattern B were 14.1%, 10.2% and 7.8% lower than those of pattern A , respectively. Grain yield of glutinous rice increased with N application rate increasing, while N use efficiency decreased with N application rate increasing. There was not significantly difference in N use efficiency of glutinous rice between pattern A and B . The results suggest that the N application rate of 120 kg/hm2 with B nitrogen application pattern is the optimal choice for glutinous rice production.