To study the steam explosion pretreatment and enzymatic saccharification technical parameters of kenaf fiber, the steam explosion pressure, pressuring time and filler amount of steam explosion were determined and the effects of bath ratio, enzymolysis time, enzyme type, enzyme dosage and enzyme ratio on enzymatic saccharification were analyzed by single factor and orthogonal experiments. The results showed that the optimum pretreatment parameters of steam explosion were as follow: packing volume 100%, steam explosion pressure 2.5 MPa, pressure holding time 240–300 s. The enzymatic saccharification parameters were as follow: bath ratio of (1∶5.00) – (1∶7.50), cellulose and xylanase ratio of 2∶3. With the mixed enzymes (1.50% cellulose+2.250% xylanase) and pH5.0, the saccharification rate was above 70%.