Water, ethyl acetate, anhydrous ethanol and petroleum ether, acetone, methanol were used to extract Capsella bursa-pastoris powder, the inhibitory effects of 6 kinds of solvent extracts on Ralstonia solanacearum were investigated through indoor virulence test , the results showed that only ethyl acetate and water extracts from Capsella bursa-pastoris had antimicrobial activity on Ralstonia solanacearum , the of ethyl acetate extract reached 63.9%, and antibacterial rate of water extract was 28.9%. The influences of water and ethyl acetate extracts on morphology, growth curve,utilization rate of sugar and protein content of Ralstonia solanacearum were investigated by transmission electron microscopy (SEM), wheat flange dividing turbidimetry, anthrone colorimetry, coomassie brilliant blue method .Electron microscopy showed that the structures of cell membrane and cell wall were changed and the bacteria cracked in Ralstonia solanacearum treated with water extract and ethyl acetate extract from Capsella bursa-pastorisof . Ethyl acetate extract and water extract from Capsella bursa-pastoris can make Ralstonia solanacearum logarithmic phase lag, reduce sugar utilization, and increase protein content in Ralstonia solanacearum.